When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

Using the procedure reported here, all the pipetting steps in LM-PCR can be performed on a Biomek 2000 liquid handling robotic workstation. After thermocycling is completed in the first reaction, the ...

A new thermocycler technology helps scientists optimize reactions with six different settable temperature zones for better control and linear gradients. The Applied Biosystems™ QuantStudio™ Real-Time ...

Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. Post editing, it is critical to provide precise and accurate quantification for ...

PCR amplification can seem straightforward, but in single-cell workflows even small miscalculations can quickly complicate results and downstream analysis. Conventional PCR systems require users to ...

The polymerase chain reaction -- A brief comparison between in vivo DNA replication and in vitro PCR amplification -- The PCR in practice -- The different types and varieties of nucleic acid target ...

This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR ...

In the summer of 1984, Henry Erlich, Glenn Horn, Randy Saiki, and myself were working on developing a DNA test for sickle cell anemia (SCA) at Cetus Corp. SCA is an autosomal dominant disease caused ...

PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...