The answer depends on the questions you’re trying to answer, as well as the origin, quantity, and quality of your samples. In this article, we’ll briefly discuss several RNA-seq workflows, how they ...

Samples are commonly stored in a way that degrades RNA. Scientists are devising new ways to overcome this obstacle for RNA sequencing. RNA is more subject to degradation than DNA, whether chemical, ...

Years of experiments and databases filled with RNA-seq results belie the simple reality that, until fairly recently, it was impossible to analyze RNA directly. The RNA-seq studies performed with ...

Traditional PCR systems require users to set a specified number of cycles according to the assay and the input amount. As a result, input material must be quantified, and samples of varying inputs ...

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RNA sequencing has emerged as a powerful supplement to DNA sequencing for Mendelian disease diagnosis, but clinical translation of diagnostic RNA-seq has not been widely achieved. Researchers at ...

RNA sequencing (RNA-seq) is a powerful tool in diagnosing Mendelian disorders, but the optimized sequencing depth for this technology has not yet been determined. In a new study published in the ...