Intrathecal Injections - Experimental Techniques - Pain Researcher
Dec 20, 2019 · Does any body have a protocol or advice for doing intrathecal injections in mice that doesn’t require surgery. Is there a way to inject directly into the lumbar enlargement (or …
Intraganglionic injections into mouse DRG? - Protocols - Pain …
Jul 11, 2016 · Have you ever heard or seen or done an intrathecal injection of an AAV construct with any neuron specific promoter which then goes to the DRG and then travels along …
Intrathecal siRNA injection for spinal neuron gene knockdown
Nov 3, 2016 · While I have never done intrathecal injections, I do a lot of nerve injections and we usually allow around 2 days for retrograde transport, after that we perform our injury and it …
Death after intrathecal capsaicin? - Pain Researcher
Nov 16, 2023 · I inject only 7’5ul per animal, which is a quantity that is in the range reported by papers that use intrathecal capsaicin at that concentrations. I though that dose was safe but …
Direct Intrathecal Injection of Recombinant Adeno-associated …
Mar 5, 2022 · Topic Replies Views Activity Intraspinal injection of adeno-associated viruses into the adult mouse spinal Experimental Techniques 0 355 September 4, 2021 Intrathecal …
Astrocytes transduction in the spinal cord - Experimental …
Aug 9, 2018 · Have one tried lentivirus for astrocytes transduction in the spinal cord? Could intrathecal injection be used? @rurongji, @achamess
Intraganglionic injections into mouse DRG? - Page 2 - Protocols
May 8, 2024 · Dear Juan Carlos, Did you finally conducted the experiment involving the intrathecal injection of AAV9-hSyn-DIO-hM3Dq-mCherry in Nav1.8-Cre animals? I am …
Systemic Administration of AAV PHP.S for Delivery to DRG
Sep 23, 2021 · Have you tried IP admin at P1? How was the transduction efficiency? There are papers showing the AAV transduction is dependent on administration routes and mouse …
EF1a promoter to drive expression in DRG in vivo?
Nov 17, 2017 · I finally tried AAV-EF1a intrathecal injections and the infection wasn’t successful in my cre animals. I tried AAV9-EF1α-mCherry-FLEX-DTA (homemade, not great titer) and …
Intraspinal injections - To Laminectomy or Not? - Pain Researcher
Jul 19, 2017 · I don’t have experience doing spinal cord injections, but the possibility of injection without laminectomy is very exciting! I’ve always been concerned with the effect of the …