2. In Sanger sequencing, only one primer, either forward or reverse, is used, whereas PCR uses both the primers.

a. reverse transcriptase b. a DNA template c. primers flanking the sequence to be amplified d. free deoxyribonucleotides (dNTPs) The following represents a DNA strand in the process of …

When preparing primers for PCR, we have a 100 mM stock solution. The experiment requires that forward and reverse primers are 20 uM each. How will you prepare the dilution? You will mix …

To conduct a Sanger sequencing reaction, what do you need to add to Eppendorf tube with the DNA you want to sequence? A RNA polymerase B. DNA polymerase C. DNA Ligase D. …

In PCR a forward primer and reverse primer are used. The reason the primers are used is for the annealing stage in PCR to bind to the target sequence... See full answer below.

What Tm value (to 1 decimal place) would you use for the reverse primer when calculating the annealing temperature for the first 5 cycles of PCR? Assume the reverse primer sequence is …

Two kinds of primers are used: forward and reverse primers. One type of primer attaches to the leading strand of DNA and the other to the lagging strand.

A pcr reaction recipe calls for 50ul total volume: the normal conditions call for 2 microliters forward and reverse primer at 5 micromoles. What happens if you add 1/4 ul, 1/2 microliter, 4 …

Reverse transcriptase (RNA dependent DNA polymerase) and primase (RNA polymerase) are both polymerases that exhibit normal polymerase behaviors. Answer and Explanation: 1 …

Polymerase Chain Reaction Primers: PCR primers are brief DNA sequences that are used to initiate the PCR process. They are made to bind to specific areas of the target DNA sequence …